Cryoultramicrotomy as a Preparative Method for X-ray Microanalysis in Pathology

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Abstract
The role of ion shifts in cell injury became one of the most intriguing fields of application of X-ray microanalysis in pathology. Cryo(ultra)microtomy is the best preparative method for X-ray microanalysis of diffusible substances. The choice of freezing method and coolant, the use of high MW polymer cryoprotectants and their possible physiological effects, the choice of the cryosectioning temperature, and techniques for handling and transfer of sections are discussed. Experiences with the sectioning of cryoprotected tissue are presented. Other preparative techniques for microanalysis are compared to cryoultramicrotomy. The tissue used included guinea pig skin, rat brown adipose tissue, liver, pancreas and submandibular gland.