EFFECT OF HYPERTHERMIA (45-DEGREES-C) ON CALCIUM FLUX IN CHINESE-HAMSTER OVARY HA-1 FIBROBLASTS AND ITS POTENTIAL ROLE IN CYTOTOXICITY AND HEAT-RESISTANCE

  • 15 July 1987
    • journal article
    • research article
    • Vol. 47  (14) , 3712-3717
Abstract
Hyperthermia caused a major increase in uptake of 45Ca2+ into Chinese hamster ovary HA-1 cells. Increased permeability to Ca2+ was observed with heating periods as brief as 45.degree. C for 4 min and reached a maximum at 45.degree. C for 30 min. In addition to elevation of Ca2+ influx, heat induced an increase in 45Ca2+ exchange with the extracellular Ca2+ pool. The effect of heat on Ca2+ permeability was transient, and Ca2+ influx returned to normal values by approximately 9 h at 37.degree. C. Comparison of the time courses of increased Ca2+ permeability and cell inactivation at 45.degree. C indicated that the heating time required for maximum permeability to Ca2+ was similar to the initial resistant "shoulder" period of the cell survival curve. This suggests that Ca2+ could play a permissive role in thermal cell inactivation; efficient cell killing may require a threshold concentration of intracellular Ca2+. The kinetics of heat-induced increase in Ca2+ permeability also resembled that for the induction of thermotolerance. This might suggest a messenger role for Ca2+ in thermotolerance induction. Direct increase in cellular Ca2+ levels with Ca2+ ionophore A23187 (5 .times. 10-6 M) led to subsequent heat resistance. However, the heat resistance produced by A23187 was of a lesser magnitude than heat-induced thermotolerance. In addition, A23187 did not induce the stress protein species characteristic of thermotolerance (heat shock proteins), but instead led to the synthesis of a related set of proteins (glucose-regulated proteins). The data thus suggest a role for Ca2+ in the cellular effects of hyperthermia. They are also of potential clinical relevance in that cellular responses to heat might be modified pharmacologically, by the judicious use of Ca2+ active agents, such as Ca2+ ionophores and channel blockers.