Attempts to Produce Identical Offspring in the Sheep by Mechanical Division of the Ovum

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Abstract
In an attempt to produce identical offspring artificially, the inner cell mass of ova, collected on days 4, 6 and 7 after oestrus (day 0 = day of oestrus), was mechanically divided into two equal sections. The sections were then either cultured in vitro for 2 days and the resulting apparently normal blasto-cysts transferred to recipient ewes or, in the case of day-7 ova, transferred to recipient ewes without a period of in vitro culture.

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