Cloning of bacterial DNA replication genes in bacteriophage λ
- 1 October 1982
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 187 (3) , 501-509
- https://doi.org/10.1007/bf00332635
Abstract
Recombinant lambda phages containing the genes for dnaZ protein (the γ subunit of DNA polymerse III holoenzyme), primase (dnaG protein) and dnaC protein from Escherichi coli and Salmonella typhimurium were isolated. Each gene cloned from S. typhimurium has extensive DNA sequence homology to the corresponding E. coli gene. Clones selected by complementation of a dnaA temperature-sensitive mutant appear similar to other isolated suppressors of dnaA (Projan and Wechsler 1981). Derivatives of each cloned fragment suitable for overproduction of the protein were constructed. Of those tested, only the phage containing the E. coli dnaZ gene resulted in significant overproduction.This publication has 33 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- ENZYME STUDIES OF ΦX174 DNA REPLICATIONPublished by Elsevier ,1980
- T4 polynucleotide ligase catalyzed joining of short synthetic DNA duplexes at base-paired endsBiochemistry, 1978
- A cold sensitive dnaA mutant of E. coli which overinitiates chromosome replication at low temperatureMolecular Genetics and Genomics, 1978
- Five Hundredfold Overproduction of DNA Ligase After Induction of a Hybrid Lambda Lysogen Constructed in VitroScience, 1977
- A colony bank containing synthetic CoI EI hybrid plasmids representative of the entire E. coli genomeCell, 1976
- Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coliJournal of Molecular Biology, 1975
- Bacteriophage ReceptorsAnnual Review of Microbiology, 1973
- On the process of cellular division in Escherichia coli: III. Thermosensitive Mutants of Escherichia coli Altered in the Process of DNA InitiationJournal of Molecular Biology, 1970
- Escherichia coli mutants with temperature-sensitive synthesis of DNAMolecular Genetics and Genomics, 1970