Improvement of Oligonucleotide Probe Design Criteria for Functional Gene Microarrays in Environmental Applications
- 1 February 2006
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 72 (2) , 1688-1691
- https://doi.org/10.1128/aem.72.2.1688-1691.2006
Abstract
To optimize oligonucleotide probe design criteria, PCR products with different similarities to probes were hybridized to a functional gene microarray designed to detect homologous genes from different organisms. In contrast to more restrictive probe designs based on a single criterion, simultaneous consideration of the percent similarity (≤90%), the length of identical sequence stretches (≤20 bases), and the binding free energy (≥−35 kcal mol −1 ) was found to be predictive of probe specificity.Keywords
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