BLASTOGENIC FACTORS - PRODUCTION BY T-LYMPHOCYTES AND B-LYMPHOCYTES EFFECT ON T-CELLS AND B-CELLS

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 33  (1) , 23-29
Abstract
The cells responsible for in vitro production of blastogenic factor were identified and the effect of blastogenic factor on T [thymus derived] and B [bone marrow derived] lymphocytes was evaluated. Human T lymphocytes were purified by a nylon wool column filtration method and B-cell-enriched populations were purified by sedimentation of E [erythrocyte] rosettes on Ficoll-Hypaque. Both T cells and B cells produced blastogenic factor. Blastogenic factor produced by T cells differed from blastogenic factor produced by B cells. Blastogenic factor produced in unmixed B cell cultures stimulated both B cells and T cells to proliferate; blastogenic factor produced in unmixed T cell cultures stimulated only B cells. The production of T cell blastogenic factor was accelerated when stimulated by allogeneic cells. The blastogenic activity of supernatants from mixed cultures of B cells was not different from that of supernatants from unmixed cultures. The significance of these findings for mixed leucocyte reaction is discussed.