Taxol production in bioreactors: Kinetics of biomass accumulation, nutrient uptake, and taxol production by cell suspensions of Taxus baccata
- 20 September 1995
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 47 (6) , 666-676
- https://doi.org/10.1002/bit.260470607
Abstract
The kinetics of biomass accumulation, nutrient uptake and taxol production of Taxus baccata cell suspensions were examined in three bioreactor configurations, viz. 250-mL Erienmeyerflasks, 1-L working volume pneumatically mixed (PMB), and stirred tank (STB) bioreactors. Qualitatively similar kinetics were observed in all three bioreactor types. Biomass accumulation and specific nutrient uptake rates exhibited biphasic characteristics. Carbohydrate uptake and biomass accumulation substantially ceased when phosphate was depleted from the medium. Phosphate was identified as a possible growth-limiting nutrient. Taxol accumulated exclusively in the second phase of growth. A maximum taxol concentration of 1.5 mg/L was obtained in the PMB which was fivefold greater than that obtained in the Erienmeyer flasks and the STB, but the relative kinetics of taxol production was the same in all three reactor types. Biomass yields were calculated from the kinetic data and a stoichiometry for biomass formation was evaluated. The similarity of kinetics in the three bioreactor configurations suggests that taxol production by T. baccata cell suspensions is amenable to scateup. © 1995 John Wiley & Sons, Inc.Keywords
This publication has 34 references indexed in Scilit:
- Bioreactor Engineering as an Enabling Technology to Tap BiodiversityAnnals of the New York Academy of Sciences, 1994
- Improved taxol yield by aromatic carboxylic acid and amino acid feeding to cell cultures of taxus cuspidataBiotechnology & Bioengineering, 1994
- Rapid Isocratic Reversed Phase HPLC of Taxanes on New Columns Developed Specifically for Taxol AnalysisJournal of Liquid Chromatography, 1993
- Effects of different nitrogen sources on glutamine synthetase and ferredoxin dependent glutamate synthase activities and on free amino acid composition in radish plantsSoil Science and Plant Nutrition, 1990
- Growth ofCatharanthus roseus cell suspensions in bioreactors: On-line analysis of oxygen and carbon dioxide levels in inlet and outlet gas streamsBiotechnology Letters, 1988
- Effect of aeration rate and influence of pCO2 in large-scale cultures ofCatharanthus roseus cellsApplied Microbiology and Biotechnology, 1986
- Growth of plant cell suspension cultures with ammonium as the sole source of nitrogenCanadian Journal of Botany, 1977
- Studies on the Growth in Culture of Plant CellsJournal of Experimental Botany, 1977
- Effect of ammonium on growth of plant cells (Ipomoea sp.) in suspension culturesCanadian Journal of Botany, 1975
- Nutrient requirements of suspension cultures of soybean root cellsExperimental Cell Research, 1968