Identification of phosphopeptides by mass spectrometry
- 29 October 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 273 (1-2) , 31-35
- https://doi.org/10.1016/0014-5793(90)81044-o
Abstract
Phosphopeptides can be identified by ion spray mass spectrometry. The method was tested with phosphokemptide and with a proteolytic digest of one subunit (δ‐subunit) of the nicotinic acetylcholine receptor. In the latter one peptide containing tyrosine phosphate, one with two serine phosphates, and two different peptides each containing one serine phosphate were unambiguously identified. Thus it is proven that ion spray mass spectrometry can be applied for the localization of phosphorylation sites in a known primary structure.Keywords
This publication has 10 references indexed in Scilit:
- Structure, oligosaccharide structures, and posttranslationally modified sites of the nicotinic acetylcholine receptor.Proceedings of the National Academy of Sciences, 1989
- Regulation of receptor function by protein phosphorylationTrends in Pharmacological Sciences, 1987
- Determination of the sites of cAMP-dependent phosphorylation on the nicotinic acetylcholine receptor.Published by Elsevier ,1987
- Ion spray interface for combined liquid chromatography/atmospheric pressure ionization mass spectrometryAnalytical Chemistry, 1987
- Determination of sulfonated azo dyes by liquid chromatography/atmospheric pressure ionization mass spectrometryAnalytical Chemistry, 1987
- Sequence analysis of phosphoserine‐containing peptidesFEBS Letters, 1986
- The reaction site of a non-competitive antagonist in the delta-subunit of the nicotinic acetylcholine receptor.The EMBO Journal, 1986
- The nicotinic acetylcholine receptor and its ion channelEuropean Journal of Biochemistry, 1986
- Membranes Rich in Acetylcholine Receptor: Characterization and Reconstitution to Excitable Membranes from Exogenous LipidsEuropean Journal of Biochemistry, 1978