ANALYSIS OF CELL CYCLE COMPARTMENTS OF HEPATOCYTES AFTER PARTIAL HEPATECTOMY

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Abstract
An analysis of cell kinetic parameters as a function of intralobular localization of hepatocytes [rat] was performed using autoradiographic methods to obtain a basis for a quantitative description of cell cycle compartments after partial hepatectomy. The influx into the S compartment revealed a maximum in a lobular zone not immediately adjacent to the portal tract, with a decrease towards the perivenous and periportal area of the lobule. The maximum influx was in the intermediate zone at 34 h and a lower one in the perivenous parts of the lobule at 40 h. The influx pattern at 56 h was similar to the situation at 18 h. The fraction of labeled mitoses in the whole liver and in subunits of the lobule disclosed a constant duration of S whereas the duration of G2 + M was prolonged from the periportal to the perivenous zone. A graphical model of kinetic events after partial hepatectomy is proposed, which describes the sizes of the various cell cycle compartments. The model successfully predicted the results of a continuous labeling experiment.