Influence of 1,2,3-Benzene-tricarboxylate on Pyruvate Metabolism in Rat-Liver Mitochondria

Publisher Website
Google Scholar
Abstract
1,2,3-Benzene-tricarboxylate, a known inhibitor of the mitochondrial tricarboxylate carrier, inhibited pyruvate carboxylation and the transport of citrate out of the matrix in rat liver mitochondria incubated with pyruvate. The inhibition of pyruvate carboxylation was observed with both intact mitochondria and solubilized pyruvate carboxylase [EC 6.4.1.1]. The inhibition of pyruvate carboxylase by 1,2,3-benzene-tricarboxylate was not mediated via one of the parameters known to regulate the activity of the enzyme and therefore a direct inhibition of the enzyme by the tricarboxylate was assumed. Since pyruvate carboxylase is exclusively localized in the mitochondrial matrix space it was concluded that 1,2,3-benzene-tricarboxylate penetrates this compartment.

This publication has 20 references indexed in Scilit: