The Relative Value of Liquid Media, Glucose Cystine Blood Agar, and Mouse Inoculation in the Titration of Pasteurella tularensis

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Abstract
P. tularensis was grown on liquid media and on glucose cystine blood agar. The liquid medium consisted of either that described by Tamura and Gibby or a modification of that described by Steinhaus, Parker and McKee. Glucose cystine blood agar was found to be capable of supporting the growth of this organism even though very small inocula were transferred to the surface of slants or petri plates containing this media. The number of organisms needed to initiate growth on this medium is comparable to the number necessary to induce infection in mice when injd. intraperit. into these animals. The number of organisms needed to initiate growth in liquid media is many times the number required to infect mice. The organism was transferred for 22 passages in modified Steinhaus medium without loss of virulence for mice.

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