Formation of Thyroglobulin-bound Iodine in Cell-free System and Effect of Thyrotropic Hormone*

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Abstract
Pig thyroid glands were homogenized with 0.25 M sucrose containing 0.002 M ethylenediaminetetraacetate and fractionated by centrifuging at 700 and 80,000 x g. The particulate fraction (mitochondria-microsomes) catalyzed the incorporation of KI-I131 to thyroglobulin at pH 7.2 in the presence of glucose oxidase-glucose system. Any other tissue proteins were scarcely iodinated. The liver particulate fraction could not replace the thyroid preparation. I131 -incorporation to thyroglobulin was inhibited by Mg2+ or Ca2+, and was accelerated by TSH at a proper concentration. In respect to the particulate concentration.

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