TWO VIRUS DISEASES OF RHUBARB IN EASTERN ONTARIO

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Abstract
Two viruses were isolated from Ruby Red rhubarb growing in the Ottawa district. Isolate I produced a severe rugosity and mottle on rhubarb, a systemic mottle in Nicotiana tabacum, N. glutinosa and Datura stramonium, and local lesions on Phaseolus vulgaris, Vigna sinensis and Beta vulgaris. The virus was inactivated after being heated for 10 minutes at 65 °C., and became non-infective after the sap was diluted to 1:2000. Isolate II incited the formation of chlorotic and necrotic spots on rhubarb, and produced a systemic mottle in N. tabacum, N. glutinosa, and D. stramonium. The virus was inactivated by being heated at 75 °C. for ten minutes, and became non-infective after the sap was diluted 1:8000. Both isolates were mechanically transmitted by diluting crude sap 1;100 in 0.1 M. phosphate buffer (pH 8–9) and rubbing on leaves dusted with carborundum. The success in isolating these viruses or virus strains was dependent on the season in which isolations were attempted. The size of the virus particles of both isolates was 478 × 15μm.

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