Application of HPLC to the Purification of Coupling Factor CF1From Spinach Chloroplasts and of Some of its Subunits
- 1 May 1987
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 10 (7) , 1507-1517
- https://doi.org/10.1080/01483918708066783
Abstract
A simple and rapid method of purification of coupling factor CF1, from spinach chloroplast crude extracts, is described, involving anion exchange HPLC on DEAE Pak. Separation of β, CF1 - δ, CF1-ε subunits is performed on the same column, after dissociation of the bound enzyme by extensive washing at low ionic strength. Purity of the fractions is checked by urea-SDS polyacrylamide electrophoresis and by fluorescence spectrometry.Keywords
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