Quantitative Determination of Cyclobenzaprine in Human Plasma by High Pressure Liquid Chromatography

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Abstract
A method is presented for the analysis of cyclo-benzaprine in human plasma utilizing high pressure liquid chromatography (HPLC) with UV detection at 230 nm. Plasma, adjusted to pH 10.5, was extracted with methylene chloride:pentane 30:70 (v/v). Nortriptyline was employed as the internal standard, and chromatographic separation was achieved with a normal-phase column. Standard curves were linear over the concentration range 1.0 ng/ml to 49.0 ng/ml. The method was utilized to assay plasma samples from 24 humans given a 20 mg oral dose of cyclobenzaprine.