DIFFERENTIATION OF YEASTS BY ELECTROPHORETIC METHODS

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Abstract
An examination of the esterase isoenzymes reacting with α-naphthyl acetate indicated five types of electrophoretic pattern in 94 strains of 54 species of 15 yeast genera. Groups classified according to esterase patterns agreed with accepted taxonomic groups, but there were no differences of obvious value for routine identification of separate species of yeasts. Strictly aerobic yeasts produced up to four bands of esterase activity; facultative anaerobes produced two lines or only one. Proteins detected by staining with trifalgic acid after electrophoresis formed identical patterns from all yeasts tested.