Identification of a carrier by using Vi enzyme-linked immunosorbent assay serology in an outbreak of typhoid fever on an Indian reservation

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Abstract
In May 1981 an outbreak of typhoid fever occurred in a small village on a southwestern USA Indian reservation. Five of 6 culture-proven cases, but only 2 of 15 community, age-matched controls, had eaten food prepared for a party held in the village on 20 April (.chi.2 = 4.3; P L 0.05). Food histories obtained from 16 persons who ate food at the party suggested that chicken with chili (P = 0.03) and potato salad (P = 0.09) were possible vehicles. Eleven adults who attended the party, 5 of whom helped prepare an implicated food, were studied with .gtoreq. 1 stool cultures and serum for Vi antibody by using enzyme-linked immunosorbent assay (ELISA) and hemagglutination techniques. All initial stool cultures were negative for Salmonella typhi; 1 subject, a 70-yr-old female foodhandler, had a Vi antibody titer of 1:320 by ELISA. Subsequent cultures from this subject were positive for S. typhi. ELISA for Vi antibody directed the investigators to a single individual as the most probable carrier source and obviated the need for multiple fecal cultures from the other potential carriers identified by the epidemiological investigation.