Isolation of Human Urinary Kallikrein by Affinity Chromatography. Determination of human urinary kallikrein, I.
- 1 January 1977
- journal article
- research article
- Published by Walter de Gruyter GmbH in cclm
- Vol. 15 (1-12) , 479-483
- https://doi.org/10.1515/cclm.1977.15.1-12.479
Abstract
Human urinary kallikrein (EC 3.4.21.8) was purified to electrophoretic homogeneity by gel filtration on Sephadex G-100, followed by affinity chromatography on aprotinin-Sepharose. The MW, estimated by gel filtration, is 64,000 daltons, by dodecyl sulfate electrophoresis 45,000 daltons and 29,000 daltons, and it is microheterogeneous on electrofocusing, yielding isoelectric point values of 3.8, 3.9 and 4.05. The kinetic constants for hydrolysis of substrates and human HMW[high molecular weight]-kininogen were determined. Benzamidine and aprotinin are competitive inhibitors of human urinary kallikrein.This publication has 26 references indexed in Scilit:
- SOME PROPERTIES OF HIGHLY PURIFIED HORSE URINARY KALLIKREIN*Annals of the New York Academy of Sciences, 2006
- Urinary Kallikrein Excretion in Normal ManCirculation Research, 1974
- Purification and properties of a human urinary kallikrein (kininogenase)Biochemistry, 1974
- Purification of rat urinary kallikreins and their specific antibodyBiochemistry, 1974
- Urinary kallikrein from normal and hypertensive ratsCellular and Molecular Life Sciences, 1973
- Effects of Mineralocorticoids, Altered Sodium Intake, and Adrenalectomy on Urinary Kallikrein in RatsCirculation Research, 1972
- RELATION BETWEEN URINARY KALLIKREIN AND RENAL FUNCTION, HYPERTENSION, AND EXCRETION OF SODIUM AND WATER IN MANThe Lancet, 1972
- RELEASE OF A PROSTAGLANDIN E-LIKE SUBSTANCE FROM CANINE KIDNEY BY BRADYKININ - COMPARISON WITH ELEDOISINCirculation Research, 1971
- Über die Kallikreinausscheidung im Harn des Menschen nach NierentransplantationKlinische Wochenschrift, 1968
- PURIFICATION AND SOME PROPERTIES OF THREE RALLIKREINSAnnals of the New York Academy of Sciences, 1963