Binding of ?-bungarotoxin and cholinergic ligands to acetylcholine receptors in the membrane of skeletal muscle

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Abstract
A membrane fraction enriched in acetylcholine receptors was prepared from denervated cat leg muscles. 97% of the alpha-bungarotoxin-binding sites in the dispersed membranes are sensitive to nicotinic cholinergic ligands. In intact msucle, 90% are. A filtration assay of the binding to the membranes of tritiated alpha-bungarotoxin, as retarded by these ligands, provides a convenient system for the determination of affinities of ligands for the muscle receptor in its membrane-bound state. Affinities were found thus for 6 nicotinic ligands. An allosteric system, rather than desensitisation, may explain the high affinities observed for certain ligands.

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