The seed globulins of 9 spp. in the family Gramineae were investigated. Two well defined globulin components, alpha and gamma, were isolated. The alpha component was found in 6 and the gamma component in 8 spp. Seeds of barley contain 2 other components, beta and delta, which were not found in any other spp. of this family. These components occur in different parts of the seed itself. Investigations on different fractions from seeds of wheat and barley have shown that the gamma component alone occurs in the embryo fraction and the alpha component in the residual fraction. The husk fraction contains gamma in higher concn. than alpha. Detns. of the mol. wt. of the purified components have given the values 29000 for alpha and 210,000 for gamma. From the pea, Pisum sativum, 2 globulin components were isolated, vicilin and legumin, first defined and investigated by Osborne. The mol. wt. of vicilin was found to be 186,000, and that of legumin 331,000. The seed globulins from 34 spp. in the family Leguminosae were investigated. Vicilin and legumin were found in all, except in some Acacia spp. and in Trifolium repens. In a few cases degradation products and complex compounds of these components have been isolated. These investigations seem to confirm the hypothesis that closely related plants contain the same seed globulins.