Uptake of Adenosine by Cultured Cerebral Vascular Smooth Muscle Cells

Abstract
Adenosine uptake by cerebral smooth muscle cells is a carrier-mediated process. The Km value for adenosine uptake is 10.0 μM and the Vmax is 0.95 nmol/ min-mg cell protein. This uptake system is inhibited by the adenosine analog 2-chloroadenosine at low adenosine concentrations. These results prove the existence of a nucleoside transport system associated with cerebral smooth muscle.