Studies on the role of carbohydrates in the adherence of Malassezia pachydermatis to canine corneocytes in vitro

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Abstract
A panel of four lectins was used to investigate the role of carbohydrates in the adherence of Malassezia pachydermatis to canine corneocytes in vitro. Pretreatment of canine corneocytes with concanavalin A (10 μg ml−1) inhibited (P < 0.01) the adherence of one out of six M. pachydermatis strains. This effect was abrogated by pre‐incubating concanavalin A with its hapten inhibitor (6% methyl α‐d‐mannopyranoside), suggesting that mannosyl‐bearing carbohydrate residues on the epithelial cells serve as ligands for adhesins expressed by this strain. However, treatment of corneocytes with soybean, wheat germ and gorse lectin, and pretreatment of the yeast cells with either of the four lectins had no reproducible effect on the adherence of two strains.