Cell surface antigens of chemically induced sarcomas of the mouse. I. Murine leukemia virus-related antigens and alloantigens on cultured fibroblasts and sarcoma cells: description of a unique antigen on BALB/c Meth A sarcoma.

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Abstract
As background for a serological definition of the unique antigens of chemically induced sarcomas, fibroblast and sarcoma cell lines of BALB/c and C57BL/6 [mouse] origin were typed by cytotoxicity and absorption tests for murine leukemia virus (MuLV)-related cell surface antigens and known alloantigens. Of the 17 cultured lines, 7 expressed the range of cell surface antigens associated with MuLV (GIX, GCSA, gp70, p30), and this was invariably associated with MuLV production. In nonproducer lines of C57BL/6 (but not BALB/c) origin, a MuLV-gp70-like molecule was found on the surface of fibroblasts and sarcoma cells. The alloantigenic phenotype of these MuLV+ and MuLV- cell lines was H-2D+, H-2K+, Thy-1.2+ or -, PC.1+ or -, Lyt-1.2-, Lyt-2.2-, Ia.7- and TL.2-. A unique antigen was defined on the BALB/c ascites sarcoma Meth A with antisera prepared in BALB/c or (BALB/c .times. C57BL/6)F1 mice. Tissue culture lines derived from this tumor were MuLV-, which facilitated serological study of the antigen. Adsorption analysis indicated that the antigen was restricted to Meth A; it could not be detected in normal or fetal BALB/c tissue, MuLV+ or MuLV- fibroblast lines, 12 syngeneic or allogeneic sarcomas or normal lymphoid cells from 13 different inbred mouse strains.
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