A Complex Between Initiation Factor IF2, Guanosine Triphosphate, and fMet-tRNA f : An Intermediate in Initiation Complex Formation
- 1 December 1971
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 68 (12) , 3122-3126
- https://doi.org/10.1073/pnas.68.12.3122
Abstract
Evidence is presented that suggests the formation of a complex between polypeptide chain-initiation factor IF 2, GTP, and fMet-tRNA(f). This complex transfers both fMet-tRNA(f) and GTP to 30S ribosomal subunits in the presence of ApUpG and initiation factor IF 1. The resultant 30S initiation complex reacts with 50S subunits to form a 70S initiation complex. fMet-tRNA(f) in this 70S complex reacts with puromycin to form fMet-puromycin. These results suggest that [IF 2, GTP, fMet-tRNA(f)] is an intermediate in the initiation of protein synthesis in Escherichia coli.Keywords
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