Mechanism of 3' splice site selection by the catalytic core of the sunY intron of bacteriophage T4: the role of a novel base-pairing interaction in group I introns.
Open Access
- 1 May 1990
- journal article
- research article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 4 (5) , 777-788
- https://doi.org/10.1101/gad.4.5.777
Abstract
The catalytic core of the sunY intron of bacteriophage T4 is separated from its 3' exon by 837 nucleotides, most of which are part of an open reading frame (ORF). Here, we report that transcripts truncated within the sunY ORF self-splice in vitro to a variety of sites in the segment immediately 3' of the core. Recognition of these proximal splice sites is shown to depend on (1) the presence on the intron side of a terminal G, which must not be part of a secondary structure; and (2) the ability of the penultimate intron nucleotide to base-pair with a 3' splice site-binding sequence (3'SSBS) located within the core. The counterpart of the 3'SSBS can be identified in most group I introns. The possible significance of such alternative splicing events for in vivo expression of intron-encoded proteins is discussed.Keywords
This publication has 32 references indexed in Scilit:
- Mitochondrial DNA sequence analysis of the cytochrome oxidase subunit II gene from Podospora anserinaJournal of Molecular Biology, 1990
- The guanosine binding site of the Tetrahymena ribozymeNature, 1989
- The catalytic core of the sunY intron of bacteriophage T4Gene, 1989
- Sequence analysis of mitochondrial DNA from Podospora anserinaJournal of Molecular Biology, 1988
- A class-I intron in a cyanelle tRNA gene from Cyanophora paradoxa: phylogenetic relationship between cyanelles and plant chloroplastsGene, 1988
- Deletion of nonconserved helices near the 3' end of the rRNA intron of Tetrahymena thermophila alters self-splicing but not core catalytic activity.Genes & Development, 1988
- 5′ exon requirement for self-splicing of the Tetrahymena thermophila pre-ribosomal RNA and identification of a cryptic 5′ splice site in the 3′ exonJournal of Molecular Biology, 1987
- One binding site determines sequence specificity of Tetrahymena pre-rRNA self-splicing, trans-splicing, and RNA enzyme activityCell, 1986
- New reactions of the ribosomal RNA precursor of Tetrahymena and the mechanism of self-splicingJournal of Molecular Biology, 1986
- Two intron sequences in yeast mitochondrial COX1 gene: Homology among URF-containing introns and strain-dependent variation in flanking exonsCell, 1983