One-step Growth Curves for Inclusion Blennorrhoea Virus in HeLa Cell Monolayers

Publisher Website
Google Scholar
Abstract
SUMMARY: When incubated in tissue-culture medium, inclusion blennorrhoea virus lost infectivity for HeLa cells at 37° but not at 30°. The rate of adsorption of virus to HeLa cell monolayers was dependent on temperature and on the volume of the inoculum. When the volume of medium was minimal, the virus was adsorbed before a detectable proportion was inactivated. Adsorption was complete after 7-8 hr. at 30° and 5-6 hr. at 37°. At 30° the intracellular virus went into eclipse and maturation was retarded. When the temperature was raised to 37° maturation was rapid so that, for practical purposes, replication was synchronized during this period. In singly infected HeLa cells, at 37° infective virus was not detected for 22-23 hr., after which the progeny increased exponentially until at 34-38 hr. 35-60 infectious particles/infected cell were formed. The total number of particles/inclusion seen in Giemsastained preparations exceeded the number of infectious units/inclusion. After 42-48 hr. infective virus was found in the supernatant and the number of intracellular infectious units began to decrease. Unlike vaccinia, another large virus, the virus progeny did not directly infect adjacent cells.