CORRELATIVE SCANNING AND TRANSMISSION ELECTRON-MICROSCOPY OF GLOMERULI
- 1 January 1977
- journal article
- research article
- Vol. 37 (6) , 569-578
Abstract
Scanning electron microscopy was performed on 125 human renal specimens and control rat kidneys in conjunction with light microscopy and transmission electron microscopy. Specimens were prepared by glomerular isolation, free hand slicing, alcohol cryofracture and polyethylene glycol embedding and ultraplaning. Heavy metal secondary electron sources on tissues were produced by osmic acid, thiocarbohydrazide, osmic acid treatment (O-T-O) followed by Au-Pd coating or by staining with uranyl acetate and lead citrate. Critical point drying was used uniformly. In diseased human glomeruli, podocyte changes were normal rat glomeruli were studies. Normal rat glomeruli were studied. In diseased human glomeruli, podocyte changes were classed as cytoplasmic projection, foot process changes and changes of necrosis or basement membrane denudation. Artifactual lesions of compression and contamination were defined. Cryofractured and ultraplaned stained specimens were very useful for studying dieased tissues. These methods offer the opportunity to increase our conceptural and diagnostic understanding of glomerular disease.This publication has 4 references indexed in Scilit:
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