Histochemical and Thin Layer Chromatographic Analyses of Neutral Lipids in Echinostoma revolutum Metacercariae Cultured In vitro

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Abstract
Excysted metacercariae of E. revolutum cultured in vitro in the defined medium, NCTC 135 supplemented with 20% hens egg yolk, doubled their mean relative body area and showed significant sucker growth within 14 days. Histochemical Oil Red O staining showed neutral fat mainly in the excretory system of excysted metacercariae and in adults grown in the domestic chick. In vitro cultured worms showed neutral fat in the intestine, parenchyma and excretory system. As detected by TLC the major neutral lipid fractions were free fatty acids for excysted metacercariae; free sterols for adults grown in chicks; and triglycerides, free fatty acids and free sterols for cultured worms. Excysted metacercariae excreted free fatty acids into a nonnutrient incubation medium, whereas cultured worms excreted diglycerides, triglycerides and free fatty acids into the medium.

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