Simultaneous Quantitation of Indole 3-Acetic Acid and Abscisic Acid in Small Samples of Plant Tissue by Gas Chromatography/Mass Spectrometry/Selected Ion Monitoring
Open Access
- 1 October 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 85 (2) , 419-422
- https://doi.org/10.1104/pp.85.2.419
Abstract
Indole 3-acetic acid (IAA) was analyzed in apple, orange, and prune tissue by GC-MS by monitoring the protonated molecular ion of its methyl ester at mass to charge ratio (m/z) 190 together with the major fragment ion at m/z 130 and the corresponding ions from the methyl esters of either [2H4]IAA (m/z 194, 134) or [2H5]IAA (m/z 195, 135). Abscisic acid (ABA) was analyzed by monitoring the major fragment ions of its methyl ester at m/z 261 and m/z 247 and the corresponding ions from the methyl ester of [2H3]ABA (m/z 264, 250). Detection limits for IAA and ABA were 1 and 10 picograms, respectively using standards and 1 nanogram per gram dry weight for both phytohormones in plant tissue.This publication has 3 references indexed in Scilit:
- Purification and Measurement of Abscisic Acid and Indoleacetic Acid by High Performance Liquid ChromatographyPlant Physiology, 1986
- Improved Procedure for the Estimation of Nanogram Quantities of Indole-3-acetic Acid in Plant Extracts using the Indolo-α-pyrone Fluorescence MethodPlant Physiology, 1980
- Synthesis of 4,5,6,7 and 2,4,5,6,7 Deuterium-labeled Indole-3-Acetic Acid for Use in Mass Spectrometric AssaysPlant Physiology, 1980