Use of Salmonella phage P22 for transduction in Escherichia coli
Open Access
- 1 November 1993
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 175 (21) , 7115-7118
- https://doi.org/10.1128/jb.175.21.7115-7118.1993
Abstract
A cosmid (pPR1347) carrying both the rfb gene cluster and the rfc gene of a Salmonella group B serovar has been constructed; Escherichia coli K-12 strains carrying this cosmid produce long-chain O antigen, are sensitive to phage P22, and can be transduced by P22. Some of the benefits of P22 transduction are now available for studying E. coli and potentially other genera.Keywords
This publication has 21 references indexed in Scilit:
- Structure and sequence of the rfb (O antigen) gene cluster of Salmonella serovar typhimurium (strain LT2)Molecular Microbiology, 1991
- Designation of Salmonella enterica sp. nov., nom. rev., as the Type and Only Species of the Genus Salmonella: Request for an OpinionInternational Journal of Systematic and Evolutionary Microbiology, 1987
- A low copy number cosmidPlasmid, 1987
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Improved in vitro packaging of coliphage lambda DNA: a one-strain system free from endogenous phageGene, 1985
- A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gelsAnalytical Biochemistry, 1982
- Electrophoretic resolution of the ‘major outer membrane protein’ of Escherichia coli K12 into four bandsFEBS Letters, 1975
- Properties of hybrids between Salmonella phage P22 and coliphage λNature, 1974
- Relation of lipopolysaccharide character to P1 sensitivity in Salmonella typhimuriumVirology, 1974
- A phage P22 gene controlling integration of prophageVirology, 1967