Simple and Efficient Vitrification Procedure for Cryopreservation of Mouse Embryos.
- 1 January 1997
- journal article
- Published by Japanese Association for Laboratory Animal Science in Experimental Animals
- Vol. 46 (3) , 231-234
- https://doi.org/10.1538/expanim.46.231
Abstract
Mouse pronuclear oocytes and 2-cell embryos derived from in vitro fertilization were cryopreserved by a novel simple vitrification procedure. Most cryopreserved oocytes/embryos were morphologically normal after warming, and 89-92% of them developed to the blastocyst stage during the culture. Moreover, the rate of morphologically normal pronuclear oocytes after being repeatedly cooled and warmed three times was as high as that of oocytes cooled and warmed only once, and 85% of them developed to the blastocyst stage. In addition, 43-57% of the cryopreserved oocytes/embryos transferred to recipients had developed normally to live fetuses observed on day 18.5 of pregnancy.Keywords
This publication has 8 references indexed in Scilit:
- Cryopreservation of expanded mouse blastocysts by vitrification in ethylene glycol-based solutionsReproduction, 1993
- A simple method for mouse embryo cryopreservation in a low toxicity vitrification solution, without appreciable loss of viabilityReproduction, 1990
- High survival rate of unfertilized mouse oocytes after vitrificationReproduction, 1989
- Ultrarapid freezing: a new low-cost and effective method of embryo cryopreservationFertility and Sterility, 1987
- Ice-free cryopreservation of mouse embryos at −196 °C by vitrificationNature, 1985
- EMBRYO BANKS IN THE FUTURE OF DEVELOPMENTAL GENETICSGenetics, 1974
- Survival of Mouse Embryos Frozen to -196° and -269°CScience, 1972
- Studies on the fertilization of mouse eggs in vitroThe Japanese journal of animal reproduction, 1971