Modulation of IL-1, tumor necrosis factor, and C5a-mediated murine neutrophil migration by alpha-melanocyte-stimulating hormone.
Open Access
- 1 March 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 142 (5) , 1646-1651
- https://doi.org/10.4049/jimmunol.142.5.1646
Abstract
This study was designed to examine the effects of i.p.-injected alpha-melanocyte stimulating hormone (MSH) on murine neutrophil migration into subcutaneously implanted sponges in response to IL-1-alpha, TNF-alpha, and C5a. The results show that as little as 0.1 ml of 5 x 10(-7) M MSH injected i.p. significantly blocked the accumulation of neutrophils in sponges in response to IL-1. This action of MSH was dose dependent, reversible, and was maximally effective if MSH was given at the same time as the injection of IL-1. This effect of MSH was not restricted to IL-1-induced neutrophil emigration, because MSH also antagonized the accumulation of neutrophils in response to both TNF and C5a. The proopiomelanocortin-derived peptide ACTH which contains the MSH sequence also significantly reduced neutrophil accumulation in response to IL-1, although less effectively than MSH. Similar studies with beta-endorphin showed that it had no effect on neutrophil accumulation in this system. The direct injection of MSH, beta-endorphin and ACTH into sponges or i.p. did not stimulate a neutrophil emigration and eliminated the possibility that MSH or ACTH suppressed the neutrophil influx in response to IL-1, TNF, or C5a by competing for circulating neutrophils. The action of MSH on IL-1, TNF, and C5a-induced neutrophil emigration suggests that this peptide may be an important regulator of the inflammatory response.This publication has 24 references indexed in Scilit:
- Chemotactic properties of partially purified human epidermal cell-derived thymocyte-activating factor (ETAF) for polymorphonuclear and mononuclear cells.The Journal of Immunology, 1983
- Antipyretic Potency of Centrally Administered α-Melanocyte Stimulating HormoneScience, 1983
- Virus-Induced Corticosterone in Hypophysectomized Mice: A Possible Lymphoid Adrenal AxisScience, 1982
- Induction of interleukin 1 secretion and enhancement of humoral immunity by binding of human C5a to macrophage surface C5a receptors.The Journal of Experimental Medicine, 1982
- The effect of fever on central α-MSH concentrations in the rabbitPeptides, 1981
- Human lymphocyte production of corticotropin and endorphin-like substances: association with leukocyte interferon.Proceedings of the National Academy of Sciences, 1981
- Central and peripheral injections of ACTH (1–24) reduce fever in adrenalectomized rabbitsPeptides, 1981
- 4-Norleucine, 7-D-phenylalanine-alpha-melanocyte-stimulating hormone: a highly potent alpha-melanotropin with ultralong biological activity.Proceedings of the National Academy of Sciences, 1980
- Central administration of peptides alters thermoregulation in the rabbitPeptides, 1980
- Primary structural analysis of the polypeptide portion of human C5a anaphylatoxin. Polypeptide sequence determination and assignment of the oligosaccharide attachment site in C5a.Journal of Biological Chemistry, 1978