Inosine 5′-phosphate dehydrogenase of pea seeds

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Abstract
Inosine 5''-phosphate dehydrogenase, which catalyses the reaction [image] was found in pea-seed extracts. Potassium ions were necessary for the reaction; NH4+ and , to a slight extent, Na+ ions could partly replace K+ ions as cofactor for the enzyme. The effects of K+ ion concentration, pH and concentration of substrates on inosine 5''-phosphate-dehydrogenase activity were studied. The enzyme was strongly inhibited by sulphydryi reagents. The rate of reaction was very small when inosine 5''-phosphate was replaced by inosine or when diphosphopyridine nucleotide was replaced by triphosphopyridine nucleotide.