The effect of Mg2+ on density of proplastids from the developing castor bean and the use of acetyl-CoA carboxylase activity for their cytochemical identification

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Abstract
Proplastids from developing castor beans, Ricinus communis L., were isolated using velocity gradient centrifugation on sucrose gradients with superimposed gradients of Mg2+. Preparations were free of contamination by mitochondria and showed only slight contamination by microbodies. The use of acetyl-CoA carboxylase as a cytochemical marker for castor-bean proplastids was demonstrated.

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