Calcium uptake and release by rat liver mitochondria in the presence of rat liver cytosol or the components of cytosol

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Abstract
A study has been made of factors present in rat liver cytosol that might regulate the calcium content of mitochondria. A cytosol preparation containing all the components of molecular weight greater than 10,000 prevented uptake and caused early release of accumulated calcium. These effects were due to free long-chain fatty acids and their coenzyme A derivatives present in the cytosol, and these inhibitory effects were controlled by inclusion of Mg2+, carnitine, and adenosine triphosphate at physiological levels in the incubation medium. Palmitoyl carnitine was a good substrate for calcium uptake and did not cause release of calcium from mitochondria. A specific fatty acid-binding protein was found in cytosol which may be the intracellular transport protein for fatty acids.