Thermostability of membrane enzymes in organic solvents
- 14 July 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 203 (1) , 41-43
- https://doi.org/10.1016/0014-5793(86)81432-6
Abstract
Two multisubunit enzymes of the inner mitochondrial membrane, cytochrome oxidase and the H+-ATPase may be transferred into highly apolar solvents as protein-lipid complexes. At 70 degrees C and an initial water concentration of 13 microliters per ml organic solvent (toluene), the half-life of the ATPase was approx. 11 h, whereas that of cytochrome oxidase was about 100 s. Thermostability of cytochrome oxidase could be increased more than 100-times by decreasing the water concentration to 3 microliters per ml toluene. At this latter concentration of water the half-life of the ATPase at 90, 80 and 70 degrees C was 5, 48 and 96 h, respectively.Keywords
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