An unusual RNA polymerase binding site in the immunity region of phage lambda
- 1 November 1980
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 180 (2) , 369-376
- https://doi.org/10.1007/bf00425850
Abstract
Summary RNA polymerase binds very tightly at a site called Brex in the λ immunity region, to the left of the rex gene and about 600 nucleotides to the right of PL. The complex formed is resistant to L M NaCl in the absence of nucleotide triphosphate. While in vitro little or no transcription is observed from Brex, in vivo, when inserted in a plasmid vector which allows detection of its activity, it acts as an efficient promoter. We have mapped the site protected by RNA polymerase against DNase and determined its sequence which is abnormal compared that of an average promoter.Keywords
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