In Vitro Formation and Development of Floral Buds on Tobacco Stem Explants

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Abstract
Stem segments were excised from plants of ''Wisconsin 38'' tobacco (Nicotiana tabacum L.) in 3 regions differing in their distance below the inflorescence. They were cultured in vitro in 8- and 16-h days. After 8 wk, floral and vegetative buds were counted, and the extent of floral development was assessed. Kinetin at 10-5 M inhibited formation and development of floral buds regardles of IAA concentration. Supplied at this concentration with adequate auxin, kinetin stimulated vegetative bud formation and may have caused floral bud abortion. IAA (.gtoreq. 10-6 M) inhibited vegetative and floral bud formation when supplied with low kinetic concentration (.ltoreq. 10-7 M) but did not affect floral development. When supplied with high kinetin concentration (.gtoreq. 10-6 M), it inhibited floral bud formation and stimulated vegetative bud formation. More floral buds were formed in 16-h days than in 8-h days. Few formed on explants other than those derived from the region nearest the inflorescence regardless of other treatment.