Comparison of manually performed Microtiter plate and semiautomated (cuvette) indirect enzyme-linked immunosorbent assays for the serodiagnosis of mycoplasmal pneumonia of swine
Open Access
- 1 September 1983
- journal article
- Published by American Veterinary Medical Association (AVMA) in American Journal of Veterinary Research
- Vol. 44 (9) , 1734-1737
- https://doi.org/10.2460/ajvr.1983.44.09.1734
Abstract
SUMMARY AND CONCLUSIONS: Standardized enzyme-linked immunosorbent assays for the serodiagnosis of mycoplasmal pneumonia of swine were developed, using the traditional Microtiter plate method and a method based on semiautomated processor-analyzer instrumentation. The results of the 2 procedures were not significantly different when the titers of positive and negative anti-Mycoplasma hyopneumoniae standard reference sera were determined in triplicate in 3 separate experiments. Although both methods yielded reproducible results, the instrument-assisted enzyme-linked immunosorbent assay would be preferable if large numbers of samples are to be tested.This publication has 0 references indexed in Scilit: