Functional map of human cytomegalovirus AD169 defined by global mutational analysis
Top Cited Papers
- 30 September 2003
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 100 (21) , 12396-12401
- https://doi.org/10.1073/pnas.1635160100
Abstract
Human cytomegalovirus has a complex double-stranded DNA genome of ≈240,000 bp that contains ≈150 ORFs likely to encode proteins, most of whose functions are not well understood. We have used an infectious bacterial artificial chromosome to introduce 413 defined insertion and substitution mutations into the human cytomegalovirus AD169 genome by random and site-directed transposon mutagenesis. Mutations were produced in all unique ORFs with a high probability of encoding proteins for which mutants have not been previously documented and in many previously characterized ORFs. The growth of selected mutants was assayed in cultured human fibroblasts, and we now recognize 41 essential, 88 nonessential, and 27 augmenting ORFs. Most essential and augmenting genes are located in the central region, and nonessential genes generally cluster near the ends of the viral genome.Keywords
This publication has 32 references indexed in Scilit:
- The human cytomegalovirus genome revisited: comparison with the chimpanzee cytomegalovirus genome FN1Journal of General Virology, 2003
- Mutant Human Cytomegalovirus Lacking the Immediate-Early TRS1 Coding Region Exhibits a Late DefectJournal of Virology, 2002
- Identification and Expression of Human Cytomegalovirus Transcription Units Coding for Two Distinct Fcγ Receptor HomologsJournal of Virology, 2002
- Open Reading Frame UL26 of Human Cytomegalovirus Encodes a Novel Tegument Protein That Contains a Strong Transcriptional Activation DomainJournal of Virology, 2002
- Human Cytomegalovirus UL47 Tegument Protein Functions after Entry and before Immediate-Early Gene ExpressionJournal of Virology, 2002
- Requirement for Uracil-DNA Glycosylase during the Transition to Late-Phase Cytomegalovirus DNA ReplicationJournal of Virology, 2001
- A Highly Efficient Escherichia coli-Based Chromosome Engineering System Adapted for Recombinogenic Targeting and Subcloning of BAC DNAGenomics, 2001
- Human Cytomegalovirus with IE-2 (UL122) Deleted Fails To Express Early Lytic GenesJournal of Virology, 2001
- Identification of the Human Cytomegalovirus G Protein-Coupled Receptor Homologue Encoded by UL33 in Infected Cells and Enveloped Virus ParticlesVirology, 1996
- Analysis of the Protein-Coding Content of the Sequence of Human Cytomegalovirus Strain AD169Published by Springer Nature ,1990