Peak Identification of the Conjugated Bile Acids in Reversed-Phase Liquid Chromatography by Using the Conjugation Selectivity

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Abstract
It has been found for the first time that the selectivity between five glycine and taurine conjugated bile acids remains constant despite a considerable variation in retention values for each pair of the compounds in reversed — phase liquid chromatography (RPLC). The dependence of parameter S on log k′w in retention equation log k′ = log k′w—Sϕ results in parallel lines for glycine and taurine conjugates. The selectivity between glycine and taurine conjugates has been utilized for the identification of the conjugated bile acids in RPLC.