βAPP gene transfer into cultured human muscle induces inclusion-body myositis aspects
- 1 July 1997
- journal article
- other
- Published by Wolters Kluwer Health in NeuroReport
- Vol. 8 (9) , 2155-2158
- https://doi.org/10.1097/00001756-199707070-00012
Abstract
Direct transfer of the beta-amyloid precursor protein (beta APP) gene into cultured normal human muscle, using recombinant adenovirus vector, was sufficient to induce several of the typical light microscopic, electron microscopic (EM), and EM-immunochemical aspects of the inclusion-body myositis (IBM) phenotype, including congophilia, clusters of amyloid-beta-positive 6-10 nm filaments, and 15-21 nm tubulofilamentous inclusions in the nuclei. Our results suggest that excessive production of intracellular beta APP may play an important role in the pathogenic cascade leading to the IBM phenotype.Keywords
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