Development of packed capillary column electrochromatography/mass spectrometry

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Abstract
The enhanced performance of electro‐osmotically driven high performance liquid chromatography (HPLC) using reversed‐phase packed capillaries has been evaluated for coupling to mass spectrometers, as an alternative to both conventional pressure‐driven elution, and to micellar electrokinetic capillary chromatography for the separation of neutral analytes. An off‐line comparison of electro‐osmotically driven versus pressure driven chromatography for the separation of an aromatic mixture shows the superiority of the method over conventional HPLC. Coupling of the technique with continuous‐flow fast‐atom bombardment for the analysis of a steroid mixture is described.