Flow cytometric analysis of immunogold cell surface label
- 1 September 1984
- Vol. 5 (5) , 543-546
- https://doi.org/10.1002/cyto.990050518
Abstract
Goat antimouse immunoglobulin antibodies conjugated to colloidal 40‐nm gold particles were used to label mouse spleen lymphocytes. The labeled cells were analysed with a flow cytometer, equipped with an argonion laser and a (0.5 mW) helium‐neon laser. The right‐angle (90°) light scatter signal of the red (632.8 nm) helium‐neon light was enhanced more than tenfold by the gold label. Dual labeling with gold and fluorescein isothiocyanate (FITC) showed no interference between the two labels. Thus immunogold provides a nonfluorescent cell surface label that can be combined with other cell labels for multiparametrical cell analysis.Keywords
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