Isolation of mutant Saccharomyces cerevisiae strains that survive without sphingolipids.
Open Access
- 1 May 1990
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 10 (5) , 2176-2181
- https://doi.org/10.1128/mcb.10.5.2176
Abstract
Sphingolipids comprise a large, widespread family of complex eucaryotic-membrane constituents of poorly defined function. The yeast Saccharomyces cerevisiae is particularly suited for studies of sphingolipid function because it contains a small number of sphingolipids and is amenable to molecular genetic analysis. Moreover, it is the only eucaryote in which mutants blocked in sphingolipid biosynthesis have been isolated. Beginning with a nonreverting sphingolipid-defective strain that requires the addition of the long-chain-base component of sphingolipids to the culture medium for growth, we isolated two strains carrying secondary, suppressor mutations that permit survival in the absence of exogenous long-chain base. Remarkably, the suppressor strains made little if any sphingolipid. A study of how the suppressor gene products compensate for the lack of sphingolipids may reveal the function(s) of these membrane lipids in yeast cells.This publication has 17 references indexed in Scilit:
- ANIMAL GLYCOSPHINGOLIPIDS AS MEMBRANE ATTACHMENT SITES FOR BACTERIAAnnual Review of Biochemistry, 1989
- One-step gene replacement in yeast by cotransformationGene, 1985
- Occurrence of novel antigenic phosphoinositol-containing sphingolipids in the pathogenic yeast Histoplasma capsulatumBiochemistry, 1984
- On the formation of alpha-hydroxy fatty acids. Evidence for a direct hydroxylation of nonhydroxy fatty acid-containing sphingolipids.Journal of Biological Chemistry, 1984
- Analysis of sphingoid bases by reversed-phase high performance liquid chromatography.Journal of Lipid Research, 1983
- The isolation and characterization of a mutant strain of Saccharomyces cerevisiae that requires a long chain base for growth and for synthesis of phosphosphingolipids.Journal of Biological Chemistry, 1983
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry, 1983
- Isolation of the yeast regulatory gene GAL4 and analysis of its dosage effects on the galactose/melibiose regulon.Proceedings of the National Academy of Sciences, 1982
- Conditional-lethal mutations that suppress genetic defects in morphogenesis by altering structural proteins.Proceedings of the National Academy of Sciences, 1975
- ISOLATION AND PARTIAL CHARACTERIZATION OF A MAJOR INOSITOL-CONTAINING LIPID IN BAKER'S YEAST, MANNOSYL-DIINOSITOL, DIPHOSPHORYL-CERAMIDEProceedings of the National Academy of Sciences, 1969