Geometric Properties of Covalently Bonded DNA on Single-Crystalline Diamond
- 1 March 2006
- journal article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 128 (12) , 3884-3885
- https://doi.org/10.1021/ja058181y
Abstract
Diamond is a promising candidate for bioapplications. Properties of hybridized DNA arrays on single-crystalline diamond are studied on a microscopic level by atomic force microscopy (AFM) in buffer solutions. Compact DNA layers in a thickness of 76 A are resolved by optimizing phase and height contrast in AFM. The height shows some long-range (30 nm) undulations of +/-5 A due to tip and DNA interactions. The axis of double helix DNA is oriented at about 36 degrees with respect to the diamond surface. DNA molecules can be removed by contact-mode AFM with forces >45 nN, indicating stronger DNA bonding than on gold substrates.Keywords
This publication has 22 references indexed in Scilit:
- Photochemical Functionalization of Hydrogen-Terminated Diamond Surfaces: A Structural and Mechanistic StudyThe Journal of Physical Chemistry B, 2005
- Spatial and Mechanical Properties of Dilute DNA Monolayers on Gold Imaged by AFMThe Journal of Physical Chemistry B, 2003
- DNA-modified nanocrystalline diamond thin-films as stable, biologically active substratesNature Materials, 2002
- Photochemical Functionalization of Diamond FilmsLangmuir, 2002
- Multiple Word DNA Computing on SurfacesJournal of the American Chemical Society, 2000
- Covalent Immobilization of Oligonucleotides on Modified Glass/Silicon Surfaces for Solid-phase DNA Hybridization and AmplificationChemistry Letters, 1998
- Alkyl Monolayers on Silicon Prepared from 1-Alkenes and Hydrogen-Terminated SiliconJournal of the American Chemical Society, 1995
- Sequence-Specific Gene Detection with a Gold Electrode Modified with DNA Probes and an Electrochemically Active DyeAnalytical Chemistry, 1994
- Multiplexed biochemical assays with biological chipsNature, 1993
- Covalent immobilization of DNA onto glassy carbon electrodesElectroanalysis, 1992