Elicitor-Induced Formation of Pterocarpan Phytoalexins in Chickpea (Cicer arietinum L.) Cell Suspension Cultures from Constitutive Isoflavone Conjugates upon Inhibition of Phenylalanine Ammonia Lyase

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Abstract
After inhibition of phenylalanine ammonia lyase by L- α-aminooxy-β-phenylpropionic acid, the constitutively formed formononetin 7-O-glucoside-6″-O-malonate is metabolized with the isoflavone aglycone being used as an intermediate in the elicitor-induced formation of pterocarpan phytoalexins in chickpea cell suspension cultures. In elicited cultures not treated with the inhibitor phytoalexins are synthesized de novo from phenylalanine. Therefore, in chickpea cells the constitutive isoflavone conjugate metabolism and the elicitor-induced pterocarpan formation show metabolic linkage under specific physiological conditions.