The effect of salt concentration on the iron-binding properties of human transferrin
- 1 March 1982
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 201 (3) , 527-532
- https://doi.org/10.1042/bj2010527
Abstract
The salt dependence of the Fe-binding properties of transferrin was studied by urea/polyacrylamide-gel electrophoresis. The distribution of Fe between the N-terminal and C-terminal binding sites under equilibrium conditions and the rates of release of FE from the 2 sites were studied. Salt increases the thermodynamic stability of Fe binding in the N-terminal site relative to the C-terminal site. Similar behavior is observed for the kinetics of Fe release, where salt retards the rate of removal of Fe from the N-terminal site but facilitates removal from the C-terminal site.This publication has 7 references indexed in Scilit:
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