Bacterial diversity of the Inner Mongolian Baer Soda Lake as revealed by 16S rRNA gene sequence analyses
- 1 October 2003
- journal article
- research article
- Published by Springer Nature in Extremophiles
- Vol. 8 (1) , 45-51
- https://doi.org/10.1007/s00792-003-0358-z
Abstract
Bacterial diversity associated with Baer Soda Lake in Inner Mongolia of China was investigated using a culture-independent method. Bacterial 16S rRNA gene libraries were generated using bacterial oligonucleotide primers, and 16S rRNA gene sequences of 58 clones were analyzed phylogenetically. The library was dominated by 16S rDNAs of Gram-negative bacteria (24% α-Proteobacteria, 31% β-Proteobacteria, 33% γ-Proteobacteria, and 2% δ-Proteobacteria), with a lower percentage of clones corresponding to Gram-positive bacteria. Forty cloned sequences were similar to that of known bacterial isolates (>97% sequence similarity), represented by the species of the genera Brevundimonas, Comamonas, Alcaligenes, Stenotrophomonas, and Klebsiella. Eighteen cloned sequences showed less affiliation with known taxa (<97% sequence similarity) and may represent novel taxa.Keywords
This publication has 28 references indexed in Scilit:
- Alcaligenes faecalis subsp. parafaecalis subsp. nov., a Bacterium Accumulating Poly-β-hydroxybutyrate from Acetone-butanol Bioprocess ResiduesSystematic and Applied Microbiology, 2001
- Phylogenetic analyses of Klebsiella species delineate Klebsiella and Raoultella gen. nov., with description of Raoultella ornithinolytica comb. nov., Raoultella terrigena comb. nov. and Raoultella planticola comb. nov.International Journal of Systematic and Evolutionary Microbiology, 2001
- Comparison of Antifungal Activities and 16S Ribosomal DNA Sequences of Clinical and Environmental Isolates of Stenotrophomonas maltophiliaJournal of Clinical Microbiology, 2001
- Expanding the Known Diversity and Environmental Distribution of an Uncultured Phylogenetic Division of BacteriaApplied and Environmental Microbiology, 2000
- Application of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus.The Journal of General and Applied Microbiology, 2000
- Novel archaeal phylotypes from an East African alkaline salternExtremophiles, 1999
- Phylogenetic identification and in situ detection of individual microbial cells without cultivation.1995
- TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environmentBioinformatics, 1994
- The neighbor-joining method: a new method for reconstructing phylogenetic trees.Molecular Biology and Evolution, 1987
- Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli.Proceedings of the National Academy of Sciences, 1978