Amino acid sequence of the heavy chain variable region from the A/J mouse antiarsonate monoclonal antibody 36-60 bearing a minor idiotype
- 1 August 1983
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 22 (18) , 4291-4296
- https://doi.org/10.1021/bi00287a020
Abstract
In addition to the predominant idiotype family (IdCR) associated with the murine A/J anti-azophenylarsonate response, a 2nd idiotype family (Id36-60) was defined on the basis of serologic cross-reactivity and amino acid sequence homology among monoclonal antibodies. The complete variable region amino acid sequence of the A/J IgG2a hybridoma protein 36-60 H chain was obtained by automated Edman degradation of the intact chain and fragments generated by cleavage with CNBr and by tryptic digestion of a succinylated CNBr peptide. A comparison of the Id36-60 H chain sequence to that of the IdCR H chain variable region reveals only 45% homology between them. Evidently, different genes encode the VH, D and JH gene-encoded sequences in the 2 idiotypes.This publication has 21 references indexed in Scilit:
- An intrastrain cross-reactive idiotype associated with anti-p-azophenylarsonate antibodies of BALB/c mice.The Journal of Immunology, 1981
- Relationship of idiotypes of the anti-p-azophenylarsonate antibodies of A/J and BALB/c mice.The Journal of Immunology, 1981
- Use of fluorescamine as an effective blocking reagent to reduce the background in protein sequence analyses by the beckman automated sequencerAnalytical Biochemistry, 1981
- Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genesNature, 1980
- Structural studies on induced antibodies with defined idiotypic specificities. IX. Framework differences in the heavy- and light-chain-variable regions of monoclonal anti-p-azophenylarsonate antibodies from A/J mice differing with respect to a cross-reactive idiotype.The Journal of Experimental Medicine, 1980
- Hybridoma proteins expressing the predominant idiotype of the antiazophenylarsonate response of A/J mice.Proceedings of the National Academy of Sciences, 1980
- Light chain variable region sequence of rabbit antipneumococcal type III polysaccharide antibody 3368Biochemistry, 1978
- Automated amino acid sequence of small peptides utilizing PolybreneAnalytical Biochemistry, 1978
- Polyquarternary amines prevent peptide loss from sequenatorsAnalytical Biochemistry, 1978
- COMPLETE SEQUENCE OF MURINE MONOCLONAL IMMUNOGLOBULIN MOPC 173 (IGG2A) - GENETIC IMPLICATIONS1976